Metabolic Comfort in Oncology and Free Amino Acids: Perspectives for the Use of Their Regulatory Actions in Physiological Concentrations

The creation methodology of pathogenetic compositions of amino acids and their derivatives on the basis of their physiological concentration for practical application in oncology of their regulatory effects discussed.


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Changes in amino acid fund of liquids and tissues of patients specifically characterize a cancer illness. Correction of intermediate metabolic changes at a cancer can be reached at use of separate amino acids or their combination.
To date, there are main levels of exploitation of biochemical (metabolic) properties of amino acids and their derivatives in clinical practice: 1. The use of amino acids or multicomponent mixtures of amino acids (mainly, essential, combined with vitamins and trace elements) for replacement therapy or shortfall of essential nutrients and proteins [1] .
2. The use of drugs on the basis of individual amino acids or their compositions, developed based on the additive functional and metabolic action, in which the "exploited" pharmacological activity (effects of activation of redox processes, reactions of energy metabolism and neutralization of xenobiotics compounds) of this class [2] .
However, the use of certain levels of L-amino acids or their compositions or shortfall implement direct pharmacological effects practically ignored their regulatory effects on metabolic processes and key metabolic reactions.
To understand the metabolic processes and vital functions of the regulatory effect of amino acids, which manifests itself under natural or near concentrations of these compounds in body fluids and tissues [3][4][5] . It is obvious that the effective use of L-amino acids or their derivatives for metabolic correction and directional changes in metabolism in pathological or extreme conditions limited accumulation of information about theme mechanisms of regulatory effects of the compounds tested at concentrations comparable to their physiological (endogenous) level [6] .

Materials and Methods
Our development methodology is based on: 1. Study of physiological concentrations of free amino acids, their derivatives, precursors and metabolites, as well as, biochemical marker parameters in healthy donors and patients with various pathologies [1,6] .
2. Creating a unified database for the studied parameters, construction of empirical mathematical model consisting of patho genic markers specific pathology and amino acid profiles [2,6] . The results and our concept is based on research of formation of free amino acids fund in biological liquids and tissues 1495 of patients with cancer of mammary gland, lungs, prostate, ovaries, bladder or digestive tract.

Results and Discussion
Numerous results of determination of amino acids and their derivatives in human body fluids and tissues [4] allowed systematization of the accumulated data and identified areas for exploitation of their metabolic effects, primarily, in laboratory diagnostics and application in clinical practice as drugs [5] .
In view of the fact that the free amino acids are represented by a wide range of related chemical structure and metabolic transformations of compounds that forms in the body fluids and tissues, amino acid found proved that quantification of their pool contributes to the diagnosis of various diseases, including hepatobiliary pathology, cardiovascular and immune systems, oncological causes, cerebrovascular pathology, alcoholism and diabetes [6] . It turned out that the vast  [2,7,9] .
To date, there are evidences of the importance of not only amino acids as building blocks for protein synthesis, but regulators of gene expression at the level of mRNA translation by mTORdependent mechanism, signaling molecules and bio-logical response modifiers, as well as, precursors of a wide range of bioregulators, which play a key role in the integration of ma-jor metabolic fluxes [8,[10][11][12][13] .
Based on the positions of metabolomics, the amino acid pool of biological fluids and tissues found 61 free amino acids evaluated as a single information unit, which is a kind of "chemical projection" of the genome, proteome realized through this approach not only develops ideas about the pool of amino acids as a dynamical system generated receipt of them from outside, but also due to endogenous synthesis, transport, degradation and excretion, and allows the identification of "key points" intermediate metabolic equilibrium shift that may reflect ratios at the individual levels of endogenous amino acids and related species (metabolic related) compounds.